De volledige chloroplast-genoomsequentie van Quercus chungii (Fagaceae)
Quercus chungii FPMetcalf, een zeldzame eik die endemisch is in Zuid-China, behoort tot de stam van de samengestelde trichomenbasis (CTB) in de sectie Cyclobalanopsis . Het volledige chloroplast-genoom van de soort werd in deze studie verzameld en geannoteerd.
Het circulaire genoom was 160.731 bp groot, met een typische quadripartiete structuur, waaronder een groot single-copy-gebied (LSC, 90.140 bp), een klein single-copy-gebied (SSC, 18.911 bp) en twee kopieën van inverted repeat-regio’s (IR’s). , 25.840 bp). Het codeerde in totaal 113 unieke genen, waaronder 79 eiwitcoderende genen, 30 tRNA-genen en vier rRNA-genen.
Genoomsequenties van humaan cytomegalovirus stam TB40/E varianten vermeerderd in fibroblasten en epitheelcellen
De komst van sequencing van het hele genoom heeft onthuld dat gewone laboratoriumstammen van humaan cytomegalovirus (HCMV) grote genetische tekortkomingen hebben als gevolg van seriële passage in fibroblasten. In het bijzonder gaat tropisme voor epitheel- en endotheelcellen verloren door mutaties die de genen UL128, UL130 of UL131A verstoren, die coderen voor subeenheden van een virion-geassocieerd pentamerisch advanced (PC) dat belangrijk is voor virale toegang tot deze cellen, maar niet voor toegang tot fibroblasten. De aan endotheelcellen aangepaste stam TB40/E heeft een relatief intact genoom en is naar voren gekomen als een laboratoriumstam die sterk lijkt op het wildtype virus. Er bestaan echter verschillende heterogene TB40/E-aandelen en gekloonde varianten die een reeks sequentie- en tropisme-eigenschappen vertonen.
Hier rapporteren we het gebruik van PacBio-sequencing om de genetische veranderingen op te helderen die plaatsvonden, zowel op consensusniveau als binnen subpopulaties, bij het passeren van een TB40 / E-voorraad op ARPE-19-epitheelcellen. De lang gelezen gegevens vergemakkelijkten ook het onderzoek van de koppeling tussen mutaties. In overeenstemming met inefficiënte ARPE-19-celinvoer, bevatte ten minste 83% van de virale genomen die aanwezig waren vóór aanpassing veranderingen die van invloed waren op pc-subeenheden. In tegenstelling, en constant met het belang van de laptop voor toegang tot endotheel- en epitheelcellen, misten genomen na aanpassing deze of aanvullende mutaties die van invloed waren op pc-subeenheden.
De sequentiegegevens onthulden ook zes enkele niet-coderende substituties in de geïnverteerde herhalingsgebieden, enkele niet-synonieme substituties in genen UL26, UL69, US28 en UL122, en een frameshift-afknotgen UL141. Van de veranderingen die van invloed zijn op eiwitcoderende regio’s, werd alleen die in UL122 sterk geselecteerd. Deze verandering, resulterend in een D390H-substitutie in het gecodeerde eiwit IE2, is eerder betrokken geweest bij het essentieel maken van een ander viraal eiwit, UL84, voor virale replicatie in fibroblasten. Deze bevinding suggereert dat IE2 en misschien zijn interacties met UL84 belangrijke functies hebben die uniek zijn voor HCMV-replicatie in epitheelcellen.

Sequentiemodificatie op aanvraag: zoek en vervang instruments voor nauwkeurige genbewerking in planten
Tot voor kort was ons vermogen om allelische diversiteit in planten te genereren beperkt tot de introductie van varianten van gedomesticeerde en wilde soorten door te kweken through ongecontroleerde recombinatie of het gebruik van chemische en fysische mutagenen-processen die respectievelijk langdurig en kostbaar zijn of geen specificiteit hebben. Genbewerking biedt een snellere en nauwkeurigere manier om nieuwe variatie te creëren, hoewel de toepassing ervan in planten werd gedomineerd door het creëren van korte insertie- en deletiemutaties die leidden tot verlies van genfunctie, voornamelijk als gevolg van de afhankelijkheid van bewerkingsresultaten op het DNA-herstelpad keuzes die intrinsiek zijn aan hogere eukaryoten.
Andere soorten bewerkingen zoals puntmutaties en nauwkeurige en vooraf ontworpen gerichte sequentie-inserties zijn zelden geïmplementeerd, ondanks het feit dat ze middelen bieden om de expressie van doelwitgenen te moduleren of om de functie en stabiliteit van hun eiwitproducten te manipuleren . De afgelopen jaren zijn er verschillende ontwikkelingen ontwikkeld om aangepaste bewerkingen te vergemakkelijken door de keuzes van reparatiepaden te reguleren of door gebruik te maken van alternatieve soorten DNA-reparatie. We hebben de komst gezien van nieuwe instruments voor het bewerken van genen die onafhankelijk zijn van DNA-dubbelstrengsbreukenreparatie en methoden die volledig onafhankelijk zijn van de DNA-reparatieprocessen van de gastheer worden steeds meer onderzocht. Met het doel een uitgebreid overzicht te geven van de state-of-the-art methodologie voor allelvervanging in planten, bespreek ik de adoptie van deze verbeteringen voor plantgenoomengineering.
Gentelling van doelsequentie-opname plaatst drie volledige genoomduplicatiegebeurtenissen in Hibiscus L. (Malvaceae)
Achtergrond: De grote diversiteit in genoomgrootte en chromosoomaantal van planten is deels te wijten aan polyploïdisatie (dwz genoomverdubbeling). De verschillen in genoomgrootte en chromosoomaantal tussen diploïde plantensoorten kunnen een venster zijn op het intrigerende fenomeen van genoomverdubbeling in het verleden, dat in de loop van de tijd kan worden verdoezeld door het proces van diploïdisatie. Het geslacht Hibiscus L. (Malvaceae) heeft een grote diversiteit aan chromosoomaantallen en een complexe genomische geschiedenis. Hibiscus is ideaal voor het onderzoeken van genomische gebeurtenissen uit het verleden, need hoewel er twee oude genoomduplicatiegebeurtenissen zijn geïdentificeerd, zullen er waarschijnlijk meer worden gevonden vanwege de diversiteit aan chromosoomaantallen. Om de geschiedenis van duplicatiegebeurtenissen van het hele genoom in Hibiscus opnieuw te beoordelen, hebben we drie alternatieve situation’s getest die verschillende polyploïdisatiegebeurtenissen beschrijven.
Resultaten: Met behulp van het vastleggen van doelsequenties hebben we een nieuwe probeset voor Hibiscus ontworpen en 87 orthologe genen gegenereerd van vier diploïde soorten. We hebben paralogen gedetecteerd in> 54% vermeende single-copy genen. 34 van deze genen werden geselecteerd voor het testen van drie verschillende situation’s voor genoomduplicatie met behulp van gentelling. Alle soorten Hibiscus die werden bemonsterd, deelden één genoomduplicatie met H. syriacus, en één volledige genoomduplicatie vond plaats langs de tak die naar H. syriacus leidde.
Conclusies: Hier bevestigden we de onafhankelijke genoomverdubbeling die eerder werd gevonden in de lijn die leidde tot H. syriacus en een gedeelde genoomverdubbeling van deze lijn en de relaxation van Hibiscus. Bovendien vonden we een voorheen onontdekte genoomduplicatie die werd gedeeld door de /Pavonia- en /Malvaviscus-clades (beide genesteld in Hibiscus) met het voorkomen van twee kopieën in wat anders single-copy genen waren. Onze resultaten benadrukken de complexiteit van genomische diversiteit in sommige plantengroepen, wat orthologische beoordeling en nauwkeurige fylogenomische gevolgtrekking moeilijk maakt.
Ontwerp-genoomsequentie van Pandrug-resistente Pseudomonas aeruginosa SPA03, geïsoleerd van een patiënt met goedaardige prostaathyperplasie
Het conceptgenoom van pandrug-resistente Pseudomonas aeruginosa-stam SPA03, die behoort tot de wereldwijde hoogrisicosequentie sort 357 (ST357) en werd geïsoleerd uit een patiënt met goedaardige prostaathyperplasie, wordt in dit rapport gepresenteerd.
De genoomassemblage werd gegenereerd door kort gelezen Illumina HiSeq-X Ten en langgelezen Oxford Nanopore Applied sciences MinION-sequentiegegevens te combineren met behulp van de Unicycler-assembler.
DL2000 Plus DNA Marker |
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MD101-01 | Vazyme | 250 μl | EUR 128.4 |
DL2000 Plus DNA Marker |
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MD101-02 | Vazyme | 500 μl | EUR 135.6 |
100bp DNA Marker Plus-50, Ready-to-use |
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D1103-050 | GenDepot | 500 ul | EUR 181.2 |
100bp DNA Marker Plus-50, Ready-to-use |
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D1103-100 | GenDepot | 2x500 ul | EUR 230.4 |
Peacock Plus Prestained Protein Marker |
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9-21531 | Biotium |
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Description: Minimum order quantity: 1 unit of 50UL |
200-2000bp DNA Marker Plus, Ready-to-use |
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GM401 | Bio Basic | 50loading, 50prep | EUR 72.53 |
100-5000bp DNA Marker Plus, Ready-to-use |
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SGM03 | Bio Basic | 50loading, 50prep | EUR 80.88 |
ACTGene? DNA Marker, 1 kb Plus Ladder, 19 Fragments, 100 loads |
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ACT-IDMWD2 | ACTGene | each | EUR 178.8 |
2K Plus DNA Marker (8 linear double-stranded DNA bands) |
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20-abx098046 | Abbexa |
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2K Plus DNA Marker (9 linear double-stranded DNA bands) |
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20-abx098047 | Abbexa |
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100bp Plus DNA Marker (12 linear double-stranded DNA bands) |
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20-abx098053 | Abbexa |
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100bp Plus DNA Marker (14 linear double-stranded DNA bands) |
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20-abx098054 | Abbexa |
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SmartCheck™ Tri-color Extended Range Plus Protein Marker, 13 bands 9-245 kDA, 2x250ul |
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PR5013-TC | Benchmark Scientific | 1 each | EUR 178.2 |
2 × Taq Plus Master Mix (Dye Plus) |
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P212-01 | Vazyme | 5 ml | EUR 159.6 |
2 × Taq Plus Master Mix (Dye Plus) |
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P212-02 | Vazyme | 15 ml | EUR 231.6 |
2 × Taq Plus Master Mix (Dye Plus) |
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P212-03 | Vazyme | 50 ml | EUR 466.8 |
2 × Taq Plus Master Mix II (Dye Plus) |
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P213-01 | Vazyme | 5 ml | EUR 160.8 |
2 × Taq Plus Master Mix II (Dye Plus) |
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P213-02 | Vazyme | 15 ml | EUR 231.6 |
2 × Taq Plus Master Mix II (Dye Plus) |
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P213-03 | Vazyme | 50 ml | EUR 466.8 |
SureClean Plus |
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BIO-37047 | Bioline | 1 x 5ml | Ask for price |
SureClean Plus |
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BIO-37048 | Bioline | 2 x 12.5ml | Ask for price |
Novel Green PLUS (20000X) |
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LD003-0500 | GeneOn | 500 µl | EUR 206.4 |
Novel Green PLUS (20000X) |
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LD003-0500L | GeneOn | 5x500 µl | EUR 877.2 |
Percoll Plus 250 Ml |
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17544502 | Scientific Laboratory Supplies | EACH | EUR 268.8 |
i-BRITE Plus |
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SF40000-10 | Neuromics | 10 ml | EUR 292.8 |
Percoll Plus 1L |
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17544501 | Scientific Laboratory Supplies | EACH | EUR 741.6 |
arium mini plus |
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WAT4280 | Scientific Laboratory Supplies | EACH | EUR 5667.6 |
Bottle Pressure Plus 250mL |
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BOT8038 | Scientific Laboratory Supplies | PK10 | EUR 273.6 |
Bottle Pressure Plus 500mL |
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BOT8040 | Scientific Laboratory Supplies | PK10 | EUR 340.8 |
Plus PCR SuperMix |
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20-abx098888 | Abbexa |
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SL4 Plus Centrifuge |
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75009912 | Scientific Laboratory Supplies | EACH | EUR 8208 |
JBScreen Plus Salts |
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M-CS-503 | MiTeGen | 24 solutions | EUR 276 |
Description: JBScreen Plus Salts |
SafeView™ Plus |
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G468 | ABM | 1.0 ml (10000X) | EUR 127.2 |
SL4R Plus Centrifuge |
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75009927 | Scientific Laboratory Supplies | EACH | EUR 13219.2 |
Surface Protein Plus |
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FSA1108 | Scientific Laboratory Supplies | PK50 | EUR 211.2 |
MagSi-NGSPREP Plus* |
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MDKT00010005 | AMSBIO | 5 mL | EUR 76.8 |
MagSi-NGSPREP Plus* |
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MDKT00010075 | AMSBIO | 75 mL | EUR 714 |
MagSi-NGSPREP Plus* |
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MDKT00010500 | AMSBIO | 500 mL | EUR 3564 |
JBScreen Plus Additives |
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M-CS-504 | MiTeGen | 24 solutions | EUR 276 |
Description: JBScreen Plus Additives |
JBScreen Plus Volatiles |
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M-CS-505 | MiTeGen | 24 solutions | EUR 276 |
Description: JBScreen Plus Volatiles |
Colorex Salmonella Plus |
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MED1234 | Scientific Laboratory Supplies | PK10 | EUR 22.8 |
Super-Q½ Plus 230V 50Hz |
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ZFSQ240P4 | Scientific Laboratory Supplies | EACH | EUR 19715.87 |
JBScreen Plus Chaotropic |
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M-CS-502 | MiTeGen | 24 solutions | EUR 276 |
Description: JBScreen Plus Chaotropic |
JBScreen Plus Kosmotropic |
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M-CS-501 | MiTeGen | 24 solutions | EUR 276 |
Description: JBScreen Plus Kosmotropic |
Accuris Taq Plus, 250 units |
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PR1000-TP-250 | Benchmark Scientific | 1 PC | EUR 201.3 |
Accuris Taq Plus, 500 units |
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PR1000-TP-500 | Benchmark Scientific | 1 PC | EUR 322.5 |
2K DNA Marker |
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20-abx098045 | Abbexa |
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5K DNA Marker |
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20-abx098048 | Abbexa |
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15K DNA Marker |
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20-abx098049 | Abbexa |
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1Kb DNA Marker |
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20-abx098050 | Abbexa |
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1Kb DNA Marker |
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D1101-050 | GenDepot | 50㎍ | EUR 157.2 |
1Kb DNA Marker |
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D1101-250 | GenDepot | 5X50㎍ | EUR 360 |
AlbuSorb™ PLUS Kit |
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APK285-100 | Biotech Support Group | 100 preps | EUR 2434.8 |
Description: Albumin Removal Kit |
AlbuSorb™ PLUS Kit |
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APK285-20 | Biotech Support Group | 20 preps | EUR 625.2 |
Description: Albumin Removal Kit |
Water Plus - Total ATP |
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FSA1090 | Scientific Laboratory Supplies | PK100 | EUR 579.6 |
AlbuVoid™ PLUS Kit |
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NP-AVK-05 | Biotech Support Group | 5 preps | EUR 486 |
Description: Albumin Removal Kit |
AlbuVoid™ PLUS Kit |
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NP-AVK10 | Biotech Support Group | 10 preps | EUR 704.4 |
Description: Albumin Removal Kit |
Sartorius Midi Plus 1-100 ml |
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PIP7592 | Scientific Laboratory Supplies | EACH | EUR 608.4 |
Pressure Plus 250mL Amber Bottle |
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BOT2272 | Scientific Laboratory Supplies | PK10 | EUR 494.4 |
AlbuTrial™ PLUS Kit |
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AVPS-05 | Biotech Support Group | 5 preps AlbuSorb™ PLUS & 5 preps AlbuVoid™ PLUS Kit | EUR 625.2 |
Description: Albumin Removal Kit |
Cellmaxin Plus, 10.5mg/ml |
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C3319-006 | GenDepot | 6x1ml | EUR 250.8 |
Cellmaxin Plus, 10.5mg/ml |
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C3319-020 | GenDepot | 20ml | EUR 375.6 |
omniDOC Plus Blue LED |
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ELE2502 | Scientific Laboratory Supplies | EACH | EUR 7887.6 |
omniDOC Plus Blue LED |
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ELE2506 | Scientific Laboratory Supplies | EACH | EUR 8018.4 |
Dual Spot PLUS System |
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MIC2516 | Scientific Laboratory Supplies | EACH | EUR 1183.2 |
IKA Matrix Delta Plus |
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SHA5030 | Scientific Laboratory Supplies | EACH | EUR 2269.2 |
100bp DNA Marker |
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20-abx098052 | Abbexa |
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100bp DNA Marker |
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D1100-050 | GenDepot | 50㎍ | EUR 198 |
100bp DNA Marker |
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D1100-250 | GenDepot | 5X50㎍ | EUR 660 |
White Marker Pen |
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PCR1376 | Scientific Laboratory Supplies | EACH | EUR 41.88 |
EZ Read 800 Plus ELISA |
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MIC6144 | Scientific Laboratory Supplies | EACH | EUR 8253.6 |
Checker Plus pH Tester |
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HI98100 | Scientific Laboratory Supplies | EACH | EUR 72 |
DL5000 DNA Marker |
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MD102-01 | Vazyme | 250 μl | EUR 128.4 |
DL5000 DNA Marker |
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MD102-02 | Vazyme | 500 μl | EUR 135.6 |
RNase Inhibitor Plus, 20-40 units |
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R2808-001 | GenDepot | 10,000 units | EUR 481.2 |
RNase Inhibitor Plus, 20-40 units |
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R2808-100 | GenDepot | 1000 unis | EUR 128.4 |
RNase Inhibitor Plus, 20-40 units |
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R2808-500 | GenDepot | 5x1000 units | EUR 303.6 |
Taq Plus DNA Polymerase |
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P201-01 | Vazyme | 250 U | EUR 147.6 |
Taq Plus DNA Polymerase |
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P201-02 | Vazyme | 1000 U | EUR 213.6 |
Taq Plus DNA Polymerase |
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P201-03 | Vazyme | 3000 U | EUR 382.8 |
DL15000 DNA Marker |
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MD103-01 | Vazyme | 250 μl | EUR 130.8 |
DL15000 DNA Marker |
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MD103-02 | Vazyme | 500 μl | EUR 138 |
Direct Tube Marker |
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PCR1414 | Scientific Laboratory Supplies | EACH | EUR 7038 |
EZ Read 800 Plus Research |
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MIC6146 | Scientific Laboratory Supplies | EACH | EUR 7503.48 |
Ecl Plus Western Blotting |
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RPN2236 | Scientific Laboratory Supplies | PK3 | EUR 631.2 |
Bacteria RNA Plus Reagent |
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R402-01 | Vazyme | 20 ml | EUR 156 |
SCLC Marker Antibody |
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V3091-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: This mAb reacts with a membrane-associated protein present in normal and malignant neuroendocrine tissues including small cell lung cancer (SCLC). It stains neural and a variable number of endocrine tissues and in the lung it reacts preferentially with SCLC and carcinoids. Its epitope is destroyed during formalin fixation. This antibody was categorized during the First International Workshop on Small Cell Lung Cancer Antigens held in London in April 1987. There are two major types of Lung Carcinoma: non-small cell, which accounts for 80% of all cases; and small cell, which accounts for roughly 20% of all lung cancers reported. The lung continues to be a customary place for cancer migration from tumors elsewhere in the body. Treatment depends on the specific cell type of the cancer, level of progression and status of the individual patient. |
SCLC Marker Antibody |
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V3091-20UG | NSJ Bioreagents | 20 ug | EUR 153.3 |
Description: This mAb reacts with a membrane-associated protein present in normal and malignant neuroendocrine tissues including small cell lung cancer (SCLC). It stains neural and a variable number of endocrine tissues and in the lung it reacts preferentially with SCLC and carcinoids. Its epitope is destroyed during formalin fixation. This antibody was categorized during the First International Workshop on Small Cell Lung Cancer Antigens held in London in April 1987. There are two major types of Lung Carcinoma: non-small cell, which accounts for 80% of all cases; and small cell, which accounts for roughly 20% of all lung cancers reported. The lung continues to be a customary place for cancer migration from tumors elsewhere in the body. Treatment depends on the specific cell type of the cancer, level of progression and status of the individual patient. |
SCLC Marker Antibody |
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V3091SAF-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: This mAb reacts with a membrane-associated protein present in normal and malignant neuroendocrine tissues including small cell lung cancer (SCLC). It stains neural and a variable number of endocrine tissues and in the lung it reacts preferentially with SCLC and carcinoids. Its epitope is destroyed during formalin fixation. This antibody was categorized during the First International Workshop on Small Cell Lung Cancer Antigens held in London in April 1987. There are two major types of Lung Carcinoma: non-small cell, which accounts for 80% of all cases; and small cell, which accounts for roughly 20% of all lung cancers reported. The lung continues to be a customary place for cancer migration from tumors elsewhere in the body. Treatment depends on the specific cell type of the cancer, level of progression and status of the individual patient. |
Thermo Scientific SL1 Plus |
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75009600 | Scientific Laboratory Supplies | EACH | EUR 5914.8 |
JBScreen Plus Complete all 5 JBScreen Plus kits for a special price |
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M-CS-506 | MiTeGen | 5 Kits | EUR 1103 |
Description: JBScreen Plus Complete all 5 JBScreen Plus kits for a special price |
Thermo Scientific SL1R Plus |
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75009630 | Scientific Laboratory Supplies | EACH | EUR 10107.6 |
Thermo Scientific SL4F Plus |
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75009951 | Scientific Laboratory Supplies | EACH | EUR 12726 |
GammaBind Plus Sepharose 5mL |
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17088601 | Scientific Laboratory Supplies | EACH | EUR 1155.6 |
Thermo Scientific SL4RF Plus |
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75009953 | Scientific Laboratory Supplies | EACH | EUR 15421.2 |
Molecular Weight Marker |
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abx098957-15ul | Abbexa | 15 ul | EUR 126 |
Lab Marker Pen Blue |
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LAB2902 | Scientific Laboratory Supplies | PK10 | EUR 90.09 |
GammaBind Plus Sepharose 25mL |
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17088602 | Scientific Laboratory Supplies | EACH | EUR 4298.4 |
GammaBind Plus Sepharose 500mL |
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17088604 | Scientific Laboratory Supplies | EACH | EUR 24270 |
Nucleoli Marker Antibody |
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V2358-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. |
Nucleoli Marker Antibody |
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V2358-20UG | NSJ Bioreagents | 20 ug | EUR 153.3 |
Description: This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. |
Nucleoli Marker Antibody |
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V2358IHC-7ML | NSJ Bioreagents | 7 ml | EUR 349.3 |
Description: This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. |
Nucleoli Marker Antibody |
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V2358SAF-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. |
Nucleoli Marker Antibody |
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V7035-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki] |
Nucleoli Marker Antibody |
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V7035-20UG | NSJ Bioreagents | 20 ug | EUR 153.3 |
Description: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki] |
Nucleoli Marker Antibody |
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V7035IHC-7ML | NSJ Bioreagents | 7 ml | EUR 349.3 |
Description: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki] |
Nucleoli Marker Antibody |
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V7035SAF-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki] |
2 × Taq Plus Master Mix |
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P211-01 | Vazyme | 5 ml | EUR 160.8 |
2 × Taq Plus Master Mix |
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P211-02 | Vazyme | 15 ml | EUR 231.6 |
2 × Taq Plus Master Mix |
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P211-03 | Vazyme | 50 ml | EUR 466.8 |
ETI 8100 Plus pH Meter Kit |
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PHM4620 | Scientific Laboratory Supplies | EACH | EUR 200.4 |
Whatman Benchkote Plus 50x60cm |
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BEN1100 | Scientific Laboratory Supplies | PK50 | EUR 158.4 |
Lab Marker Pen Green |
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LAB2904 | Scientific Laboratory Supplies | PK10 | EUR 90.09 |
Oligodendrocyte Marker O1 |
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MO15001 | Neuromics | 50 ug | EUR 397.2 |
Oligodendrocyte Marker O4 |
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MO15002 | Neuromics | 50 ug | EUR 404.4 |
RotoFlex Plus Magnetic Platform |
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ARG1640 | Scientific Laboratory Supplies | EACH | EUR 712.8 |
Slide Superfrost Plus 76x26x1mm |
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MIC3040 | Scientific Laboratory Supplies | PK72 | EUR 112.28 |
Myeloid Cell Marker (Macrophage / Granulocyte Marker); Clone BM-1 (Concentrate) |
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RA0400-C.1 | ScyTek Laboratories | 0.1 ml | EUR 150 |
Myeloid Cell Marker (Macrophage / Granulocyte Marker); Clone BM-1 (Concentrate) |
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RA0400-C.5 | ScyTek Laboratories | 0.5 ml | EUR 360 |
Clear Seal Plus Sheets125mmX78mm |
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PCR1386 | Scientific Laboratory Supplies | PK100 | EUR 103.2 |
mini plus PreTreatment Cartridge |
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WAT4288 | Scientific Laboratory Supplies | EACH | EUR 417.6 |
E. coli MurA Assay Kit Plus-100 |
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MURA100KE | ProFoldin | 100 assays | EUR 444.91 |
Description: This product includes the reagents for100 assays in a 384-well plate assay format including 0.400 ml of 10 x Assay buffer, 0.035 ml of 100 x PEP, 0.035 ml of 100 x UDP-N-acetyl glucosamine (UGN), 0.035 ml of 100 x E. coli MurA (5000 nM) and 5 ml of Dye for phosphate detection. |
E. coli MurA Assay Kit Plus-500 |
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MURA500KE | ProFoldin | 500 assays | EUR 1491.11 |
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Assay buffer, 0.170 ml of 100 x PEP, 0.170 ml of 100 x UDP-N-acetyl glucosamine (UGN), 0.170 ml of 100 x E. coli MurA (5000 nM) and 25 ml of Dye for phosphate detection. |
E. coli MurC Assay Kit Plus-100 |
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MURC100KE | ProFoldin | 100 assays | EUR 444.91 |
Description: This product includes the reagents for100 assays in a 384-well plate assay format including 0.400 ml of 10 x Assay buffer, 0.035 ml of 100 x UDP-MurNAc, 0.035 ml of 100 x L-Ala, 0.035 ml of 100 x ATP , 0.035 ml of 100 x E. coli MurC (2000 nM) and 5 ml of Dye for phosphate detection. |
E. coli MurC Assay Kit Plus-500 |
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MURC500KE | ProFoldin | 500 assays | EUR 1491.11 |
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Assay buffer, 0.170 ml of 100 x UDP-MurNAc, 0.170 ml of 100 x L-Ala, 0.170 ml of 100 x ATP , 0.170 ml of 100 x E. coli MurC (2000 nM) and 25 ml of Dye for phosphate detection. |
E. coli MurD Assay Kit Plus-100 |
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MURD100KE | ProFoldin | 100 assays | EUR 444.91 |
Description: This product includes the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x UDP-MurNAc-L-Ala (UMA), 0.035 ml of 100 x D-Glu, 0.035 ml of 100 x ATP , 0.035ml of 100 x E. coli MurD (2000 nM) and 5 ml of Dye for phosphate detection. |
E. coli MurD Assay Kit Plus-500 |
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MURD500KE | ProFoldin | 500 assays | EUR 1491.11 |
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x UDP-MurNAc-L-Ala (UMA), 0.170 ml of 100 x D-Glu, 0.170 ml of 100 x ATP , 0.170 ml of 100 x E. coli MurD (2000 nM) and 25 ml of Dye for phosphate detection. |
E. coli MurE Assay Kit Plus-100 |
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MURE100KE | ProFoldin | 100 assays | EUR 444.91 |
Description: This product includes the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x UDP-MurNAc-L-Ala-D-Glu (UDP-MurNAc-dipeptide, UMAG), 0.035 ml of 100 x DAP, 0.035 ml of 100 x ATP , 0.035 ml of 100 x E. coli MurE (5000 nM) and 5 ml of Dye for phosphate detection. |
E. coli MurE Assay Kit Plus-500 |
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MURE500KE | ProFoldin | 500 assays | EUR 1491.11 |
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x UDP-MurNAc-L-Ala-D-Glu (UDP-MurNAc-dipeptide, UMAG), 0.170 ml of 100 x DAP, 0.170 ml of 100 x ATP , 0.170 ml of 100 x E. coli MurE (5000 nM) and 25 ml of Dye for phosphate detection. |
E. coli MurF Assay Kit Plus-100 |
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MURF100KE | ProFoldin | 100 assays | EUR 444.91 |
Description: This product includes the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x UDP-MurNAc-tripeptide (UMAG-DAP), 0.035 ml of 100 x D-Ala-D-Ala, 0.035 ml of 100 x ATP , 0.010 ml of 1000 x E. coli MurF (5000 nM) and 5 ml of Dye for phosphate detection. |
E. coli MurF Assay Kit Plus-500 |
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MURF500KE | ProFoldin | 500 assays | EUR 1491.11 |
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x UDP-MurNAc-tripeptide (UMAG-DAP), 0.170 ml of 100 x D-Ala-D-Ala, 0.170 ml of 100 x ATP , 0.020 ml of 1000 x E. coli MurF (5000 nM) and 25 ml of Dye for phosphate detection. |
E. coli GlmU Assay Kit Plus-100 |
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GLU100KE | ProFoldin | 100 assays | EUR 444.91 |
Description: This product includes the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer,0.0 35 ml of 100 x Enzyme Substrate, 0.035 ml of 100 x E. coli GlmU (500 nM), 0.035 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 5 ml of Dye for phosphate detection. |
E. coli GlmU Assay Kit Plus-500 |
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GLU500KE | ProFoldin | 500 assays | EUR 1491.11 |
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x Enzyme Substrate, 0.170 ml of 100 x E. coli GlmU (500 nM), 0.170 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 25 ml of Dye for phosphate detection. |
E. coli IspD Assay Kit Plus-100 |
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ISPD100KE | ProFoldin | 100 assays | EUR 444.91 |
Description: This product includes the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x MEP, 0.035 ml of 100 x CTP, 0.035 ml of 100 x E. coli IspD (200 nM), 0.035 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 5 ml of Dye for phosphate detection. |
E. coli IspD Assay Kit Plus-500 |
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ISPD500KE | ProFoldin | 500 assays | EUR 1491.11 |
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer,0.170 ml of 100 x MEP, 0.170 ml of 100 x CTP, 0.170 ml of 100 x E. coli IspD (200 nM), 0.170 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 25 ml of Dye for phosphate detection. |
Linear pRYG DNA Marker |
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TG2028-1 | TopoGen | 10 ug | EUR 214.8 |
Granulocyte Marker Antibody |
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V2339-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. |
Granulocyte Marker Antibody |
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V2339-20UG | NSJ Bioreagents | 20 ug | EUR 153.3 |
Description: This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. |
Granulocyte Marker Antibody |
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V2339IHC-7ML | NSJ Bioreagents | 7 ml | EUR 349.3 |
Description: This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. |
Granulocyte Marker Antibody |
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V2339SAF-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. |
Mitochondrial Marker(113-1) Antibody |
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BNC470739-100 | Biotium | 100uL | EUR 238.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF647 conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC470739-500 | Biotium | 500uL | EUR 652.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF647 conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC400739-100 | Biotium | 100uL | EUR 238.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF640R conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC400739-500 | Biotium | 500uL | EUR 652.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF640R conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC550739-100 | Biotium | 100uL | EUR 238.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF555 conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC550739-500 | Biotium | 500uL | EUR 652.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF555 conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC430739-100 | Biotium | 100uL | EUR 238.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF543 conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC430739-500 | Biotium | 500uL | EUR 652.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF543 conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC800739-100 | Biotium | 100uL | EUR 238.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF680 conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC800739-500 | Biotium | 500uL | EUR 652.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF680 conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC810739-100 | Biotium | 100uL | EUR 238.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF680R conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC810739-500 | Biotium | 500uL | EUR 652.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF680R conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC610739-100 | Biotium | 100uL | EUR 238.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF660R conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNC610739-500 | Biotium | 500uL | EUR 652.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), CF660R conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNUM0739-50 | Biotium | 50uL | EUR 474 |
Description: Primary antibody against Mitochondrial Marker(113-1), 1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNCB0739-100 | Biotium | 100uL | EUR 238.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), Biotin conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNCB0739-500 | Biotium | 500uL | EUR 652.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), Biotin conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNCP0739-250 | Biotium | 250uL | EUR 459.6 |
Description: Primary antibody against Mitochondrial Marker(113-1), PerCP conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNCA0739-250 | Biotium | 250uL | EUR 459.6 |
Description: Primary antibody against Mitochondrial Marker(113-1), APC conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNCAP0739-100 | Biotium | 100uL | EUR 238.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
Mitochondrial Marker(113-1) Antibody |
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BNCAP0739-500 | Biotium | 500uL | EUR 652.8 |
Description: Primary antibody against Mitochondrial Marker(113-1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |