De volledige chloroplast-genoomsequentie van Quercus chungii (Fagaceae)

Quercus chungii  FPMetcalf, een zeldzame eik die endemisch is in Zuid-China, behoort tot de stam van de samengestelde trichomenbasis (CTB) in de   sectie Cyclobalanopsis . Het volledige chloroplast-genoom van de soort werd in deze studie verzameld en geannoteerd.

Het circulaire genoom was 160.731 bp groot, met een typische quadripartiete structuur, waaronder een groot single-copy-gebied (LSC, 90.140 bp), een klein single-copy-gebied (SSC, 18.911 bp) en twee kopieën van inverted repeat-regio’s (IR’s). , 25.840 bp). Het codeerde in totaal 113 unieke genen, waaronder 79 eiwitcoderende genen, 30 tRNA-genen en vier rRNA-genen.

Genoomsequenties van humaan cytomegalovirus stam TB40/E varianten vermeerderd in fibroblasten en epitheelcellen

De komst van sequencing van het hele genoom heeft onthuld dat gewone laboratoriumstammen van humaan cytomegalovirus (HCMV) grote genetische tekortkomingen hebben als gevolg van seriële passage in fibroblasten. In het bijzonder gaat tropisme voor epitheel- en endotheelcellen verloren door mutaties die de genen UL128, UL130 of UL131A verstoren, die coderen voor subeenheden van een virion-geassocieerd pentamerisch advanced (PC) dat belangrijk is voor virale toegang tot deze cellen, maar niet voor toegang tot fibroblasten. De aan endotheelcellen aangepaste stam TB40/E heeft een relatief intact genoom en is naar voren gekomen als een laboratoriumstam die sterk lijkt op het wildtype virus. Er bestaan ​​echter verschillende heterogene TB40/E-aandelen en gekloonde varianten die een reeks sequentie- en tropisme-eigenschappen vertonen.

Hier rapporteren we het gebruik van PacBio-sequencing om de genetische veranderingen op te helderen die plaatsvonden, zowel op consensusniveau als binnen subpopulaties, bij het passeren van een TB40 / E-voorraad op ARPE-19-epitheelcellen. De lang gelezen gegevens vergemakkelijkten ook het onderzoek van de koppeling tussen mutaties. In overeenstemming met inefficiënte ARPE-19-celinvoer, bevatte ten minste 83% van de virale genomen die aanwezig waren vóór aanpassing veranderingen die van invloed waren op pc-subeenheden. In tegenstelling, en constant met het belang van de laptop voor toegang tot endotheel- en epitheelcellen, misten genomen na aanpassing deze of aanvullende mutaties die van invloed waren op pc-subeenheden.

De sequentiegegevens onthulden ook zes enkele niet-coderende substituties in de geïnverteerde herhalingsgebieden, enkele niet-synonieme substituties in genen UL26, UL69, US28 en UL122, en een frameshift-afknotgen UL141. Van de veranderingen die van invloed zijn op eiwitcoderende regio’s, werd alleen die in UL122 sterk geselecteerd. Deze verandering, resulterend in een D390H-substitutie in het gecodeerde eiwit IE2, is eerder betrokken geweest bij het essentieel maken van een ander viraal eiwit, UL84, voor virale replicatie in fibroblasten. Deze bevinding suggereert dat IE2 en misschien zijn interacties met UL84 belangrijke functies hebben die uniek zijn voor HCMV-replicatie in epitheelcellen.

Sequentiemodificatie op aanvraag: zoek en vervang instruments voor nauwkeurige genbewerking in planten

Tot voor kort was ons vermogen om allelische diversiteit in planten te genereren beperkt tot de introductie van varianten van gedomesticeerde en wilde soorten door te kweken through ongecontroleerde recombinatie of het gebruik van chemische en fysische mutagenen-processen die respectievelijk langdurig en kostbaar zijn of geen specificiteit hebben. Genbewerking biedt een snellere en nauwkeurigere manier om nieuwe variatie te creëren, hoewel de toepassing ervan in planten werd gedomineerd door het creëren van korte insertie- en deletiemutaties die leidden tot verlies van genfunctie, voornamelijk als gevolg van de afhankelijkheid van bewerkingsresultaten op het DNA-herstelpad keuzes die intrinsiek zijn aan hogere eukaryoten.

Andere soorten bewerkingen zoals puntmutaties en nauwkeurige en vooraf ontworpen gerichte sequentie-inserties zijn zelden geïmplementeerd, ondanks het feit dat ze middelen bieden om de expressie van doelwitgenen te moduleren of om de functie en stabiliteit van hun eiwitproducten te manipuleren . De afgelopen jaren zijn er verschillende ontwikkelingen ontwikkeld om aangepaste bewerkingen te vergemakkelijken door de keuzes van reparatiepaden te reguleren of door gebruik te maken van alternatieve soorten DNA-reparatie. We hebben de komst gezien van nieuwe instruments voor het bewerken van genen die onafhankelijk zijn van DNA-dubbelstrengsbreukenreparatie en methoden die volledig onafhankelijk zijn van de DNA-reparatieprocessen van de gastheer worden steeds meer onderzocht. Met het doel een uitgebreid overzicht te geven van de state-of-the-art methodologie voor allelvervanging in planten, bespreek ik de adoptie van deze verbeteringen voor plantgenoomengineering.

Gentelling van doelsequentie-opname plaatst drie volledige genoomduplicatiegebeurtenissen in Hibiscus L. (Malvaceae)

Achtergrond:  De grote diversiteit in genoomgrootte en chromosoomaantal van planten is deels te wijten aan polyploïdisatie (dwz genoomverdubbeling). De verschillen in genoomgrootte en chromosoomaantal tussen diploïde plantensoorten kunnen een venster zijn op het intrigerende fenomeen van genoomverdubbeling in het verleden, dat in de loop van de tijd kan worden verdoezeld door het proces van diploïdisatie. Het geslacht Hibiscus L. (Malvaceae) heeft een grote diversiteit aan chromosoomaantallen en een complexe genomische geschiedenis. Hibiscus is ideaal voor het onderzoeken van genomische gebeurtenissen uit het verleden, need hoewel er twee oude genoomduplicatiegebeurtenissen zijn geïdentificeerd, zullen er waarschijnlijk meer worden gevonden vanwege de diversiteit aan chromosoomaantallen. Om de geschiedenis van duplicatiegebeurtenissen van het hele genoom in Hibiscus opnieuw te beoordelen, hebben we drie alternatieve situation’s getest die verschillende polyploïdisatiegebeurtenissen beschrijven.

Resultaten:  Met behulp van het vastleggen van doelsequenties hebben we een nieuwe probeset voor Hibiscus ontworpen en 87 orthologe genen gegenereerd van vier diploïde soorten. We hebben paralogen gedetecteerd in> 54% vermeende single-copy genen. 34 van deze genen werden geselecteerd voor het testen van drie verschillende situation’s voor genoomduplicatie met behulp van gentelling. Alle soorten Hibiscus die werden bemonsterd, deelden één genoomduplicatie met H. syriacus, en één volledige genoomduplicatie vond plaats langs de tak die naar H. syriacus leidde.

Conclusies:  Hier bevestigden we de onafhankelijke genoomverdubbeling die eerder werd gevonden in de lijn die leidde tot H. syriacus en een gedeelde genoomverdubbeling van deze lijn en de relaxation van Hibiscus. Bovendien vonden we een voorheen onontdekte genoomduplicatie die werd gedeeld door de /Pavonia- en /Malvaviscus-clades (beide genesteld in Hibiscus) met het voorkomen van twee kopieën in wat anders single-copy genen waren. Onze resultaten benadrukken de complexiteit van genomische diversiteit in sommige plantengroepen, wat orthologische beoordeling en nauwkeurige fylogenomische gevolgtrekking moeilijk maakt.

Ontwerp-genoomsequentie van Pandrug-resistente Pseudomonas aeruginosa SPA03, geïsoleerd van een patiënt met goedaardige prostaathyperplasie

Het conceptgenoom van pandrug-resistente Pseudomonas aeruginosa-stam SPA03, die behoort tot de wereldwijde hoogrisicosequentie sort 357 (ST357) en werd geïsoleerd uit een patiënt met goedaardige prostaathyperplasie, wordt in dit rapport gepresenteerd.

De genoomassemblage werd gegenereerd door kort gelezen Illumina HiSeq-X Ten en langgelezen Oxford Nanopore Applied sciences MinION-sequentiegegevens te combineren met behulp van de Unicycler-assembler.

DL2000 Plus DNA Marker
MD101-01	Vazyme	250 μl	EUR 128.4

DL2000 Plus DNA Marker
MD101-02	Vazyme	500 μl	EUR 135.6

100bp DNA Marker Plus-50, Ready-to-use
D1103-050	GenDepot	500 ul	EUR 181.2

100bp DNA Marker Plus-50, Ready-to-use
D1103-100	GenDepot	2x500 ul	EUR 230.4

Peacock Plus Prestained Protein Marker
9-21531	Biotium	EUR 240.00 EUR 139.20	500UL 50UL
Description: Minimum order quantity: 1 unit of 50UL

200-2000bp DNA Marker Plus, Ready-to-use
GM401	Bio Basic	50loading, 50prep	EUR 72.53

100-5000bp DNA Marker Plus, Ready-to-use
SGM03	Bio Basic	50loading, 50prep	EUR 80.88

ACTGene? DNA Marker, 1 kb Plus Ladder, 19 Fragments, 100 loads
ACT-IDMWD2	ACTGene	each	EUR 178.8

2K Plus DNA Marker (8 linear double-stranded DNA bands)
20-abx098046	Abbexa	EUR 309.60 EUR 226.80	2.5 ml 500 ul

2K Plus DNA Marker (9 linear double-stranded DNA bands)
20-abx098047	Abbexa	EUR 309.60 EUR 226.80	2.5 ml 500 ul

100bp Plus DNA Marker (12 linear double-stranded DNA bands)
20-abx098053	Abbexa	EUR 326.40 EUR 226.80	2.5 ml 500 ul

100bp Plus DNA Marker (14 linear double-stranded DNA bands)
20-abx098054	Abbexa	EUR 360.00 EUR 243.60	2.5 ml 500 ul

SmartCheck™ Tri-color Extended Range Plus Protein Marker, 13 bands 9-245 kDA, 2x250ul
PR5013-TC	Benchmark Scientific	1 each	EUR 178.2

2 × Taq Plus Master Mix (Dye Plus)
P212-01	Vazyme	5 ml	EUR 159.6

2 × Taq Plus Master Mix (Dye Plus)
P212-02	Vazyme	15 ml	EUR 231.6

2 × Taq Plus Master Mix (Dye Plus)
P212-03	Vazyme	50 ml	EUR 466.8

2 × Taq Plus Master Mix II (Dye Plus)
P213-01	Vazyme	5 ml	EUR 160.8

2 × Taq Plus Master Mix II (Dye Plus)
P213-02	Vazyme	15 ml	EUR 231.6

2 × Taq Plus Master Mix II (Dye Plus)
P213-03	Vazyme	50 ml	EUR 466.8

SureClean Plus
BIO-37047	Bioline	1 x 5ml	Ask for price

SureClean Plus
BIO-37048	Bioline	2 x 12.5ml	Ask for price

Novel Green PLUS (20000X)
LD003-0500	GeneOn	500 µl	EUR 206.4

Novel Green PLUS (20000X)
LD003-0500L	GeneOn	5x500 µl	EUR 877.2

Percoll Plus 250 Ml
17544502	Scientific Laboratory Supplies	EACH	EUR 268.8

i-BRITE Plus
SF40000-10	Neuromics	10 ml	EUR 292.8

Percoll Plus 1L
17544501	Scientific Laboratory Supplies	EACH	EUR 741.6

arium mini plus
WAT4280	Scientific Laboratory Supplies	EACH	EUR 5667.6

Bottle Pressure Plus 250mL
BOT8038	Scientific Laboratory Supplies	PK10	EUR 273.6

Bottle Pressure Plus 500mL
BOT8040	Scientific Laboratory Supplies	PK10	EUR 340.8

Plus PCR SuperMix
20-abx098888	Abbexa	EUR 292.80 EUR 560.40	1 ml 5 ml

SL4 Plus Centrifuge
75009912	Scientific Laboratory Supplies	EACH	EUR 8208

JBScreen Plus Salts
M-CS-503	MiTeGen	24 solutions	EUR 276
Description: JBScreen Plus Salts

SafeView™ Plus
G468	ABM	1.0 ml (10000X)	EUR 127.2

SL4R Plus Centrifuge
75009927	Scientific Laboratory Supplies	EACH	EUR 13219.2

Surface Protein Plus
FSA1108	Scientific Laboratory Supplies	PK50	EUR 211.2

MagSi-NGSPREP Plus*
MDKT00010005	AMSBIO	5 mL	EUR 76.8

MagSi-NGSPREP Plus*
MDKT00010075	AMSBIO	75 mL	EUR 714

MagSi-NGSPREP Plus*
MDKT00010500	AMSBIO	500 mL	EUR 3564

JBScreen Plus Additives
M-CS-504	MiTeGen	24 solutions	EUR 276
Description: JBScreen Plus Additives

JBScreen Plus Volatiles
M-CS-505	MiTeGen	24 solutions	EUR 276
Description: JBScreen Plus Volatiles

Colorex Salmonella Plus
MED1234	Scientific Laboratory Supplies	PK10	EUR 22.8

Super-Q½ Plus 230V 50Hz
ZFSQ240P4	Scientific Laboratory Supplies	EACH	EUR 19715.87

JBScreen Plus Chaotropic
M-CS-502	MiTeGen	24 solutions	EUR 276
Description: JBScreen Plus Chaotropic

JBScreen Plus Kosmotropic
M-CS-501	MiTeGen	24 solutions	EUR 276
Description: JBScreen Plus Kosmotropic

Accuris Taq Plus, 250 units
PR1000-TP-250	Benchmark Scientific	1 PC	EUR 201.3

Accuris Taq Plus, 500 units
PR1000-TP-500	Benchmark Scientific	1 PC	EUR 322.5

2K DNA Marker
20-abx098045	Abbexa	EUR 309.60 EUR 226.80	2.5 ml 500 ul

5K DNA Marker
20-abx098048	Abbexa	EUR 309.60 EUR 226.80	2.5 ml 500 ul

15K DNA Marker
20-abx098049	Abbexa	EUR 326.40 EUR 226.80	2.5 ml 500 ul

1Kb DNA Marker
20-abx098050	Abbexa	EUR 309.60 EUR 226.80	2.5 ml 500 ul

1Kb DNA Marker
D1101-050	GenDepot	50㎍	EUR 157.2

1Kb DNA Marker
D1101-250	GenDepot	5X50㎍	EUR 360

AlbuSorb™ PLUS Kit
APK285-100	Biotech Support Group	100 preps	EUR 2434.8
Description: Albumin Removal Kit

AlbuSorb™ PLUS Kit
APK285-20	Biotech Support Group	20 preps	EUR 625.2
Description: Albumin Removal Kit

Water Plus - Total ATP
FSA1090	Scientific Laboratory Supplies	PK100	EUR 579.6

AlbuVoid™ PLUS Kit
NP-AVK-05	Biotech Support Group	5 preps	EUR 486
Description: Albumin Removal Kit

AlbuVoid™ PLUS Kit
NP-AVK10	Biotech Support Group	10 preps	EUR 704.4
Description: Albumin Removal Kit

Sartorius Midi Plus 1-100 ml
PIP7592	Scientific Laboratory Supplies	EACH	EUR 608.4

Pressure Plus 250mL Amber Bottle
BOT2272	Scientific Laboratory Supplies	PK10	EUR 494.4

AlbuTrial™ PLUS Kit
AVPS-05	Biotech Support Group	5 preps AlbuSorb™ PLUS & 5 preps AlbuVoid™ PLUS Kit	EUR 625.2
Description: Albumin Removal Kit

Cellmaxin Plus, 10.5mg/ml
C3319-006	GenDepot	6x1ml	EUR 250.8

Cellmaxin Plus, 10.5mg/ml
C3319-020	GenDepot	20ml	EUR 375.6

omniDOC Plus Blue LED
ELE2502	Scientific Laboratory Supplies	EACH	EUR 7887.6

omniDOC Plus Blue LED
ELE2506	Scientific Laboratory Supplies	EACH	EUR 8018.4

Dual Spot PLUS System
MIC2516	Scientific Laboratory Supplies	EACH	EUR 1183.2

IKA Matrix Delta Plus
SHA5030	Scientific Laboratory Supplies	EACH	EUR 2269.2

100bp DNA Marker
20-abx098052	Abbexa	EUR 326.40 EUR 226.80	2.5 ml 500 ul

100bp DNA Marker
D1100-050	GenDepot	50㎍	EUR 198

100bp DNA Marker
D1100-250	GenDepot	5X50㎍	EUR 660

White Marker Pen
PCR1376	Scientific Laboratory Supplies	EACH	EUR 41.88

EZ Read 800 Plus ELISA
MIC6144	Scientific Laboratory Supplies	EACH	EUR 8253.6

Checker Plus pH Tester
HI98100	Scientific Laboratory Supplies	EACH	EUR 72

DL5000 DNA Marker
MD102-01	Vazyme	250 μl	EUR 128.4

DL5000 DNA Marker
MD102-02	Vazyme	500 μl	EUR 135.6

RNase Inhibitor Plus, 20-40 units
R2808-001	GenDepot	10,000 units	EUR 481.2

RNase Inhibitor Plus, 20-40 units
R2808-100	GenDepot	1000 unis	EUR 128.4

RNase Inhibitor Plus, 20-40 units
R2808-500	GenDepot	5x1000 units	EUR 303.6

Taq Plus DNA Polymerase
P201-01	Vazyme	250 U	EUR 147.6

Taq Plus DNA Polymerase
P201-02	Vazyme	1000 U	EUR 213.6

Taq Plus DNA Polymerase
P201-03	Vazyme	3000 U	EUR 382.8

DL15000 DNA Marker
MD103-01	Vazyme	250 μl	EUR 130.8

DL15000 DNA Marker
MD103-02	Vazyme	500 μl	EUR 138

Direct Tube Marker
PCR1414	Scientific Laboratory Supplies	EACH	EUR 7038

EZ Read 800 Plus Research
MIC6146	Scientific Laboratory Supplies	EACH	EUR 7503.48

Ecl Plus Western Blotting
RPN2236	Scientific Laboratory Supplies	PK3	EUR 631.2

Bacteria RNA Plus Reagent
R402-01	Vazyme	20 ml	EUR 156

SCLC Marker Antibody
V3091-100UG	NSJ Bioreagents	100 ug	EUR 349.3
Description: This mAb reacts with a membrane-associated protein present in normal and malignant neuroendocrine tissues including small cell lung cancer (SCLC). It stains neural and a variable number of endocrine tissues and in the lung it reacts preferentially with SCLC and carcinoids. Its epitope is destroyed during formalin fixation. This antibody was categorized during the First International Workshop on Small Cell Lung Cancer Antigens held in London in April 1987. There are two major types of Lung Carcinoma: non-small cell, which accounts for 80% of all cases; and small cell, which accounts for roughly 20% of all lung cancers reported. The lung continues to be a customary place for cancer migration from tumors elsewhere in the body. Treatment depends on the specific cell type of the cancer, level of progression and status of the individual patient.

SCLC Marker Antibody
V3091-20UG	NSJ Bioreagents	20 ug	EUR 153.3
Description: This mAb reacts with a membrane-associated protein present in normal and malignant neuroendocrine tissues including small cell lung cancer (SCLC). It stains neural and a variable number of endocrine tissues and in the lung it reacts preferentially with SCLC and carcinoids. Its epitope is destroyed during formalin fixation. This antibody was categorized during the First International Workshop on Small Cell Lung Cancer Antigens held in London in April 1987. There are two major types of Lung Carcinoma: non-small cell, which accounts for 80% of all cases; and small cell, which accounts for roughly 20% of all lung cancers reported. The lung continues to be a customary place for cancer migration from tumors elsewhere in the body. Treatment depends on the specific cell type of the cancer, level of progression and status of the individual patient.

SCLC Marker Antibody
V3091SAF-100UG	NSJ Bioreagents	100 ug	EUR 349.3
Description: This mAb reacts with a membrane-associated protein present in normal and malignant neuroendocrine tissues including small cell lung cancer (SCLC). It stains neural and a variable number of endocrine tissues and in the lung it reacts preferentially with SCLC and carcinoids. Its epitope is destroyed during formalin fixation. This antibody was categorized during the First International Workshop on Small Cell Lung Cancer Antigens held in London in April 1987. There are two major types of Lung Carcinoma: non-small cell, which accounts for 80% of all cases; and small cell, which accounts for roughly 20% of all lung cancers reported. The lung continues to be a customary place for cancer migration from tumors elsewhere in the body. Treatment depends on the specific cell type of the cancer, level of progression and status of the individual patient.

Thermo Scientific SL1 Plus
75009600	Scientific Laboratory Supplies	EACH	EUR 5914.8

JBScreen Plus Complete all 5 JBScreen Plus kits for a special price
M-CS-506	MiTeGen	5 Kits	EUR 1103
Description: JBScreen Plus Complete all 5 JBScreen Plus kits for a special price

Thermo Scientific SL1R Plus
75009630	Scientific Laboratory Supplies	EACH	EUR 10107.6

Thermo Scientific SL4F Plus
75009951	Scientific Laboratory Supplies	EACH	EUR 12726

GammaBind Plus Sepharose 5mL
17088601	Scientific Laboratory Supplies	EACH	EUR 1155.6

Thermo Scientific SL4RF Plus
75009953	Scientific Laboratory Supplies	EACH	EUR 15421.2

Molecular Weight Marker
abx098957-15ul	Abbexa	15 ul	EUR 126

Lab Marker Pen Blue
LAB2902	Scientific Laboratory Supplies	PK10	EUR 90.09

GammaBind Plus Sepharose 25mL
17088602	Scientific Laboratory Supplies	EACH	EUR 4298.4

GammaBind Plus Sepharose 500mL
17088604	Scientific Laboratory Supplies	EACH	EUR 24270

Nucleoli Marker Antibody
V2358-100UG	NSJ Bioreagents	100 ug	EUR 349.3
Description: This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Nucleoli Marker Antibody
V2358-20UG	NSJ Bioreagents	20 ug	EUR 153.3
Description: This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Nucleoli Marker Antibody
V2358IHC-7ML	NSJ Bioreagents	7 ml	EUR 349.3
Description: This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Nucleoli Marker Antibody
V2358SAF-100UG	NSJ Bioreagents	100 ug	EUR 349.3
Description: This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Nucleoli Marker Antibody
V7035-100UG	NSJ Bioreagents	100 ug	EUR 349.3
Description: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]

Nucleoli Marker Antibody
V7035-20UG	NSJ Bioreagents	20 ug	EUR 153.3
Description: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]

Nucleoli Marker Antibody
V7035IHC-7ML	NSJ Bioreagents	7 ml	EUR 349.3
Description: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]

Nucleoli Marker Antibody
V7035SAF-100UG	NSJ Bioreagents	100 ug	EUR 349.3
Description: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]

2 × Taq Plus Master Mix
P211-01	Vazyme	5 ml	EUR 160.8

2 × Taq Plus Master Mix
P211-02	Vazyme	15 ml	EUR 231.6

2 × Taq Plus Master Mix
P211-03	Vazyme	50 ml	EUR 466.8

ETI 8100 Plus pH Meter Kit
PHM4620	Scientific Laboratory Supplies	EACH	EUR 200.4

Whatman Benchkote Plus 50x60cm
BEN1100	Scientific Laboratory Supplies	PK50	EUR 158.4

Lab Marker Pen Green
LAB2904	Scientific Laboratory Supplies	PK10	EUR 90.09

Oligodendrocyte Marker O1
MO15001	Neuromics	50 ug	EUR 397.2

Oligodendrocyte Marker O4
MO15002	Neuromics	50 ug	EUR 404.4

RotoFlex Plus Magnetic Platform
ARG1640	Scientific Laboratory Supplies	EACH	EUR 712.8

Slide Superfrost Plus 76x26x1mm
MIC3040	Scientific Laboratory Supplies	PK72	EUR 112.28

Myeloid Cell Marker (Macrophage / Granulocyte Marker); Clone BM-1 (Concentrate)
RA0400-C.1	ScyTek Laboratories	0.1 ml	EUR 150

Myeloid Cell Marker (Macrophage / Granulocyte Marker); Clone BM-1 (Concentrate)
RA0400-C.5	ScyTek Laboratories	0.5 ml	EUR 360

Clear Seal Plus Sheets125mmX78mm
PCR1386	Scientific Laboratory Supplies	PK100	EUR 103.2

mini plus PreTreatment Cartridge
WAT4288	Scientific Laboratory Supplies	EACH	EUR 417.6

E. coli MurA Assay Kit Plus-100
MURA100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reagents for100 assays in a 384-well plate assay format including 0.400 ml of 10 x Assay buffer, 0.035 ml of 100 x PEP, 0.035 ml of 100 x UDP-N-acetyl glucosamine (UGN), 0.035 ml of 100 x E. coli MurA (5000 nM) and 5 ml of Dye for phosphate detection.

E. coli MurA Assay Kit Plus-500
MURA500KE	ProFoldin	500 assays	EUR 1491.11
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Assay buffer, 0.170 ml of 100 x PEP, 0.170 ml of 100 x UDP-N-acetyl glucosamine (UGN), 0.170 ml of 100 x E. coli MurA (5000 nM) and 25 ml of Dye for phosphate detection.

E. coli MurC Assay Kit Plus-100
MURC100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reagents for100 assays in a 384-well plate assay format including 0.400 ml of 10 x Assay buffer, 0.035 ml of 100 x UDP-MurNAc, 0.035 ml of 100 x L-Ala, 0.035 ml of 100 x ATP , 0.035 ml of 100 x E. coli MurC (2000 nM) and 5 ml of Dye for phosphate detection.

E. coli MurC Assay Kit Plus-500
MURC500KE	ProFoldin	500 assays	EUR 1491.11
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Assay buffer, 0.170 ml of 100 x UDP-MurNAc, 0.170 ml of 100 x L-Ala, 0.170 ml of 100 x ATP , 0.170 ml of 100 x E. coli MurC (2000 nM) and 25 ml of Dye for phosphate detection.

E. coli MurD Assay Kit Plus-100
MURD100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x UDP-MurNAc-L-Ala (UMA), 0.035 ml of 100 x D-Glu, 0.035 ml of 100 x ATP , 0.035ml of 100 x E. coli MurD (2000 nM) and 5 ml of Dye for phosphate detection.

E. coli MurD Assay Kit Plus-500
MURD500KE	ProFoldin	500 assays	EUR 1491.11
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x UDP-MurNAc-L-Ala (UMA), 0.170 ml of 100 x D-Glu, 0.170 ml of 100 x ATP , 0.170 ml of 100 x E. coli MurD (2000 nM) and 25 ml of Dye for phosphate detection.

E. coli MurE Assay Kit Plus-100
MURE100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x UDP-MurNAc-L-Ala-D-Glu (UDP-MurNAc-dipeptide, UMAG), 0.035 ml of 100 x DAP, 0.035 ml of 100 x ATP , 0.035 ml of 100 x E. coli MurE (5000 nM) and 5 ml of Dye for phosphate detection.

E. coli MurE Assay Kit Plus-500
MURE500KE	ProFoldin	500 assays	EUR 1491.11
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x UDP-MurNAc-L-Ala-D-Glu (UDP-MurNAc-dipeptide, UMAG), 0.170 ml of 100 x DAP, 0.170 ml of 100 x ATP , 0.170 ml of 100 x E. coli MurE (5000 nM) and 25 ml of Dye for phosphate detection.

E. coli MurF Assay Kit Plus-100
MURF100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x UDP-MurNAc-tripeptide (UMAG-DAP), 0.035 ml of 100 x D-Ala-D-Ala, 0.035 ml of 100 x ATP , 0.010 ml of 1000 x E. coli MurF (5000 nM) and 5 ml of Dye for phosphate detection.

E. coli MurF Assay Kit Plus-500
MURF500KE	ProFoldin	500 assays	EUR 1491.11
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x UDP-MurNAc-tripeptide (UMAG-DAP), 0.170 ml of 100 x D-Ala-D-Ala, 0.170 ml of 100 x ATP , 0.020 ml of 1000 x E. coli MurF (5000 nM) and 25 ml of Dye for phosphate detection.

E. coli GlmU Assay Kit Plus-100
GLU100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer,0.0 35 ml of 100 x Enzyme Substrate, 0.035 ml of 100 x E. coli GlmU (500 nM), 0.035 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 5 ml of Dye for phosphate detection.

E. coli GlmU Assay Kit Plus-500
GLU500KE	ProFoldin	500 assays	EUR 1491.11
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x Enzyme Substrate, 0.170 ml of 100 x E. coli GlmU (500 nM), 0.170 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 25 ml of Dye for phosphate detection.

E. coli IspD Assay Kit Plus-100
ISPD100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x MEP, 0.035 ml of 100 x CTP, 0.035 ml of 100 x E. coli IspD (200 nM), 0.035 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 5 ml of Dye for phosphate detection.

E. coli IspD Assay Kit Plus-500
ISPD500KE	ProFoldin	500 assays	EUR 1491.11
Description: This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer,0.170 ml of 100 x MEP, 0.170 ml of 100 x CTP, 0.170 ml of 100 x E. coli IspD (200 nM), 0.170 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 25 ml of Dye for phosphate detection.

Linear pRYG DNA Marker
TG2028-1	TopoGen	10 ug	EUR 214.8

Granulocyte Marker Antibody
V2339-100UG	NSJ Bioreagents	100 ug	EUR 349.3
Description: This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas.

Granulocyte Marker Antibody
V2339-20UG	NSJ Bioreagents	20 ug	EUR 153.3
Description: This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas.

Granulocyte Marker Antibody
V2339IHC-7ML	NSJ Bioreagents	7 ml	EUR 349.3
Description: This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas.

Granulocyte Marker Antibody
V2339SAF-100UG	NSJ Bioreagents	100 ug	EUR 349.3
Description: This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas.

Mitochondrial Marker(113-1) Antibody
BNC470739-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF647 conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC470739-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF647 conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC400739-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF640R conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC400739-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF640R conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC550739-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF555 conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC550739-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF555 conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC430739-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF543 conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC430739-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF543 conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC800739-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF680 conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC800739-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF680 conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC810739-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF680R conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC810739-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF680R conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC610739-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF660R conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNC610739-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against Mitochondrial Marker(113-1), CF660R conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNUM0739-50	Biotium	50uL	EUR 474
Description: Primary antibody against Mitochondrial Marker(113-1), 1mg/mL

Mitochondrial Marker(113-1) Antibody
BNCB0739-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against Mitochondrial Marker(113-1), Biotin conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNCB0739-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against Mitochondrial Marker(113-1), Biotin conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNCP0739-250	Biotium	250uL	EUR 459.6
Description: Primary antibody against Mitochondrial Marker(113-1), PerCP conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNCA0739-250	Biotium	250uL	EUR 459.6
Description: Primary antibody against Mitochondrial Marker(113-1), APC conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNCAP0739-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against Mitochondrial Marker(113-1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Mitochondrial Marker(113-1) Antibody
BNCAP0739-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against Mitochondrial Marker(113-1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL